![]() sessile) and bract glabrous, except margin ciliate (Table 1, Figs 3– 4). However, the new species differs from the latter by smaller corolla (3.2–3.5 mm), bract stalked (ca. feddei, sharing features, such as two long and three short calyx teeth with spinescent apices, white villous outside of the calyx, acute leaves apex, obtuse serrate leave margin and villous leaves. In morphology, the putative new species is most similar to Elsholtzia feddei f. Results Morphological comparisonĭetailed morphological comparisons between the new species and four other sympatric or morphologically similar taxa are summarised in Table 1. Hard incongruence was defined as BS ≥ 80% and/or PP ≥ 0.95 ( Pelser et al. Incongruences amongst different datasets (combined ptDNA dataset, combined nrDNA dataset) were explored through visual comparison of tree topologies and support values. For each species, genomic DNA (m > 1 μg, c > 12.5 ng/μl) was sheared (yielding 50% majority rule consensus tree after discarding the first 25% of samples as burn-in. Genomic DNA was extracted from approximately 20 mg of silica-gel-dried leaves using DNA Plantzol Reagent (Hangzhou Lifefeng Biotechnology Co., Ltd.) according to the manufacturer’s protocol. Two samples of the new species were sequenced using the genome skimming approach, then 14 sequences were mined for the phylogenetic analyses. The red stars indicate the recorded localities of E. Most sequences were downloaded from GenBank, except for the new species, which was newly sequenced in the present study. The GenBank accession numbers are listed in Suppl. A total of 20 individuals, representing 16 species of Elsholtzia, were sampled, with Elsholtzia densa Benth. The nuclear (ETS, ITS) and plastid ( rbcL, matK, trnL-F, ycf1, ycf1- rps15) regions were used for reconstructing the phylogeny of the new species and its related taxa ( Li et al. Voucher specimens were deposited in Wenzhou University ( WZUH). zhongyangii were collected from Yajiang County, Sichuan Province, China from September 2012 to December 2021 (Fig. ![]() Taxon sampling and molecular analysesĪ total of 48 individuals of E. Elsholtzia specimens, collected from Sichuan, were checked in the Herbaria of CDBI, CDCM, CQNW, HITBC, HNWP, HZU, IBK, IBSC, KUN, NAS, PE, SM, SZ, TIE, WCSBG, WUK and WZUH (acronyms as in Thiers 2021). The morphological characters were examined, based on the living plants and specimens. Materials and methods Morphological study After carefully checking specimens and literature, together with evidence from molecular phylogenetic analyses, based on combined nrDNA (ETS, ITS) and combined ptDNA ( rbcL, matK, trnL-F, ycf1, ycf1- rps15) datasets, we demonstrated that it is, indeed, a new species which is described and illustrated here. villous, especially on veins abaxially, glabrous adaxially) and bract stalked (ca. 4.5–5.3 mm), bract glabrous, with a ciliate margin (vs. feddei in calyx (villous, with two long and three short teeth with spinescent apices) and leaf apices acute, but differs from the latter by its smaller corolla (3.2–3.5 mm vs. More than 60% of the Chinese species have been widely used as traditional medicinal herbs, nectar source plants, vegetables and spices ( Huang 1977 Li and Hedge 1994 Editorial Board of Zhong Hua Ben Cao 1999).ĭuring our botanical expedition to Yajiang County, Sichuan Province in September 2012, we discovered an unknown species of Elsholtzia. Many species of Elsholtzia are important plant resources used both as medicine and flavouring. Elsholtzia is mainly distributed in East Asia, with 35 species found in China ( Huang 1977 Li and Hedge 1994 Harley et al. The genus is characterised by verticillasters in continuous or interrupted spikes or capitula, compact spikes cylindric or secund, and two-lipped corolla ( Huang 1977) ( Huang 1977). Elsholtzia Willdenow ( Lamiaceae) is a member of the tribe Elsholtzieae ( Harley et al.
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